#4561 VOLUNTARY EXERCISE EXACERBATES CHRONIC KIDNEY DISEASE INDUCED BY FOLIC ACID IN MALE C57BL6J MICE

Abstract Background and Aims Voluntary exercise is of utmost importance to avert a plethora diseases ameliorate other conditions, such as neuromuscular diseases, however in some occasions, the inflammation can be exacerbated by intense physical activity. The folic acid kidney disease model key interest since it non-invasive leads conspicuous fibrosis. Our main objective was determine influence voluntary on chronic acid-treated mice. Method Male C57/Bl6 mice were divided into 3 groups, vehicle (V), (FA), + (FA E). animals kept cages with or without wheels (sedentary exercised groups) for 30 days, then received single injection (240 mg/kg i.p.) 0.3 mol/L sodium bicarbonate. euthanized after 28 days. Results Chronic exercise. decreases survival level treated (V- 100%, FA- 85,71%, FA+ E- 60%). On day administration urea levels vs. FA E P= 0,003) creatinine (V p= 0,0004; Fa+ 0,0001). Renal injury marker KIM-1 Ex 0,0131, 0,0213) higher that went wheel, but NGAL showed no significant differences. Inflammation markers IL-6 0,0337 0,0254) TLR4 <0,0001 <0,0001) well apoptosis relation BAX/BCL2 <0,0001). Picrosirius red staining used assess tubulointerstitial fibrosis architecture; from FA+E group highest destruction architecture. Conclusion We established previous has deleterious effects nephrotoxic acid–Induced CKD.